Purification and characterization of esterase enzyme isolated from contaminated soil sample

S.Chithra; K.Silambarasi; I.Yasmin

Volume : VIII, Issue : XI, November - 2019

Purification and characterization of esterase enzyme isolated from contaminated soil sample

S. Chithra, K. Silambarasi, I. Yasmin

Abstract :

Isolation and partial purification of esterase from contaminated soil sample was the main aim of this study. The production medium for organism was optimized by using different pH, Temperature, Carbon and Nitrogen sources. The esterase enzyme was highly active and stable from pH 5.0 to 9.0 with an optimum at pH 9. Its optimum temperature was 35°C. The best carbon and nitrogen sources were mannitol and yeast extract. Esterase was partially purified by ammonium sulphate precipitation, dialysis. The specific activity of partially purified esterase obtained from ammonium sulphate fractionation is found to be 8.6485U/mg and the fractionation is 5.4 fold purified from the crude enzyme preparation yielding 17.5U/mg from the crude protein. This result showed that Bacillus subtilis under study is a good producer of extra cellular esterase, which can be beneficial for industries.