Correlation of serological marker and platelet count for early Dengue detection in Bundelkhand Region

Sushil Kumar; Anil Kumar; Roma Goyal

Volume : VIII, Issue : VI, June - 2019

Correlation of serological marker and platelet count for early Dengue detection in Bundelkhand Region

Sushil Kumar, Anil Kumar, Roma Goyal

Abstract :

Background : The dengue virus causes one of the most important mosquito borne viral diseases and belong to the Flaviviridae family. Dengue infection produces a spectrum of clinical illness, range from asymptomatic to more severe such as dengue shock syndrome and dengue haemorrhagic fever. Detection of the NS1 protein (antigen) is an important marker in the diagnosis of acute dengue fever. Platelet count is only non–dengue parameter which supports the diagnosis of the dengue shock syndrome and dengue haemorrhagic fever. Aim: The aim of the study was to correlate the platelet count with dengue specific immunological parameters (NS1 antigen & IgM antibody) by the ELISA method. Material and Methods: Blood sample collected from the patient presenting with dengue like symptoms whom IgM antibody and NS1 antigen test was requested during the period of January 2018 to December 2018 was included in the study. A total 1040 serum samples separated from blood samples. The platelet count was noted in dengue positive and dengue negative cases. Result: In this study total of 1040 blood samples were collected from suspected Dengue fever patients studied in the Microbiology laboratory from the different wards (IPD) and OPD in the M.L.B. Medical College Jhansi (a tertiary care hospital based study). MLB Medical College, Jhansi is the oldest medical college of Bundelkhand and the demographic data represent whole of this region. One hundred eighty four samples tested positive for one or more Dengue specific parameters. We analyzed the association of thrombocytopenia with dengue parameter positivity. In a total 184 cases, thrombocytopenia observed in cases 86 (46.7%). Out of 130 that were positive for NS1 antigen, thrombocytopenia was seen in 86 cases (66.1%). We found that there was no significant difference (p= 0.178) between two parameter in relation to thrombocytopenia