Aim: To analyze the effect of iron deficiency anemia on glycated hemoglobin (HbA1c) in non diabetic Indians. Material and methods: 100 randomly selected non–diabetic patients with confirmed iron deficiency anemia were compared with 100 randomly selected cases without diabetes and without Iron deficiency anemia healthy adults. Results: The mean HbA1c of cases was 5.89% while it was 5.01% in healthy control. Application of statistical test shows that the difference of HbA1c between case and control group was statistically significant (p<0.01) indicating statistically higher HbA1c in cases group. Conclusion: Our results showed that iron deficiency was associated with higher proportions of HbA1c, which could cause problems in the diagnosis of uncontrolled diabetes mellitus in iron–deficient patients.