Introduction: The clinical use of antibiotics and other antimicrobial agents, as adjuvant for the treatment of periodontitis, has been extensively investigated in the past decade. Recently, special attention has been paid to natural medication, and propolis has been reported to possess certain medicinal properties. Chlorhexidine has proved to be the most effective agent for reducing plaque and gingivitis. Aim of this study is to comparatively evaluate antimicrobial efficacy of Propolis mouthwash with 0.2 % chlorhexidine on P.Gingivalis, T. Forshythia, P. Intermedia and A.a. periodontal pathogens. Materials and methods: Sterilized nutrient agar plates were inoculated by rubbing sterile cotton swabs dipped into bacterial suspensions of P.Gingivalis, T. Forshythia, P. Intermedia and A.a. (over night cultures grown at 37° on nutrient agar) over the entire surface of the plate. The micro–organisms used were cultured and made available from the strains present with the institute. The plates were allowed to culture over night at 37°. After inoculation, 10 mm diameter five wells were cut into the surface of the agar using a sterile cork borer for each sample. Propolis and 0.2% chlorhexidine, were added into wells in four different plates containing above mentioned four different bacterias Results: Result of the study showed that 100% and 75% propolis concentration showed significant antimicrobial property against P.gingivalis, T.forsythia, P.Intermedia and A.a, equal to that of chlorhexidine. Conclusion: This study showed that propolis has significant antimicrobial efficacy against periodontopathogens, in which 100% and 75% propolis showed significant antimicrobial efiicacy, which is equal to that of 0.2% chlorhexidine.