Introduction: Thrombotic occlusion of a stenosed atherosclerotic vessel is one of the main causes of ischemic stroke. Platelets play a pivotal role in atherothrombotic events during evolution of acute ischemic stroke. Mean Platelet volume (MPV) is a variable of platelet function with larger platelet being more reactive. Aims and objectives: To determine the association between incidence of ischemic stroke and MPV, platelet count. Material and methods: The study was conducted in 50 cases with age, sex matched controls between December 2018 to November 2019 at ASRAM Medical College, Eluru, Andhra Pradesh. Inclusion criteria: All patients more than 18yrs of age presenting with ischemic stroke less than 48hrs of onset. Exclusion criteria: Haemorrhagic stroke, duration of stroke more than 48hrs, patients having diseases and/or taking drugs causing platelet abnormalities Results: MPV in cases was 7.45±0.81fL and in controls was 6.84±0.59fL (p value <0.005) Platelet count in cases and controls was 2.6±0.5lakh/ µL and 2.7±0.85lakh/µL respectively (p value >0.005) Conclusion: Increased MPV is independently associated with increased incidence of acute ischemic stroke.