Background: Dengue is one of the common vectors borne viral infection with a serious threat of transfusion transmission particularly in dengue endemic areas. Aim: To find the seroprevalence of dengue virus among voluntary blood donors in Chennai. Materials and Methods: 110 blood samples from VBD were collected in 1 year period from August 2014 to July 2015 and were subjected to dengue NS1, IgM and IgG serological tests using InBios dengue ELISA kits. Results: Among 110 VBD 91.8% of the donors were positive for anti– DENV IgG and none were positive for NS1 antigen or anti–DENV IgM. Conclusion: There is a high seroprevalence of anti–DENV IgG (91.8%) in blood donors. All donor samples in this study were seronegative for dengue NS1 and IgM