Histoplasmosis is a granulomatous disease of worldwide distribution caused by a dimorphic fungus Histoplasma capsulatum. Histoplasmosis is infrequently reported in Asia and Europe. Primary laryngeal histoplasmosis is a rare disease that may mimic tuberculosis or even laryngeal cancer, and must be considered in the differential diagnosis of a patient with hoarseness and moreover in the differential diagnosis of tumors in the vocal folds, causing hoarseness. Other differential diagnosis of histoplasmosis includes paracoccidiomycosis, leishmaniasis, blastomycosis, leprosy, syphilis, actinomycosis and lymphoma. We reported an unusual case of laryngeal histoplasmosis in a man aged 50 years who presented with provisional diagnosis of malignancy.