Chronic supparative otitis media (CSOM) is a common clinical health problem and topical antibiotic is the main treatment, however the emergence of antibiotics resistant strains is leading to failure. Periodic evaluation of microbiological profile and antimicrobial sensitivity pattern of bacteria are essential for optimum medical management of CSOM patients and prevention of antibiotic resistance remains a big challenge. In our study, chronic suppurative otitis media occurred mainly in the age group of 10–19 years. Pseudomonas aeruginosa and Staphylococcus aureus were found to be most common cause of CSOM in this study. The isolated microorganism had high susceptibility rates to polymixin B, amikacin in Pseudomonas aeruginosa and vancomycin in Staphylococcus aureus isolates.