Urinary tract infection is one of the common infections in the Indian community. Distribution and susceptibility of UTI–causing pathogens change according to time and place. This study was conducted to determine the distribution and antimicrobial susceptibility of uropathogens in the Indian community as well as to determine the effect of gender and age on the etiology of bacterial uropathogens. Clean catch midstream urine samples were collected from 288 patients of the age ranging from 20 to ≥54 years. Antimicrobial susceptibility was performed on all isolated bacteria by Kirby Bauer’s disc diffusion method. The multiple antibiotic resistances (MAR) index of each antibiotic was calculated. The UTI prevalence was 42.85% in patients; however, the prevalence was significantly higher in females than in males (females: 65.47%; males: 47.24%; ). In the notable absence of studies in the treatment of uncomplicated UTI we recommend empirical antimicrobial treatment based on local sensitivities which should be changed once the local pattern of sensitivities is known