SERUM PROTEOME ANALYSIS AND IDENTIFICATION OF SURROGATE PROTEIN MARKERS IN MALARIA

Tundwal Vijay Kumar; Satyaveer Singh; Somveer; Jinger Naveen Kumar; Meena Manoj Kumar; Kochar Aditya; Tundwal Divyanshi; Kochar Sanjay Kumar

Volume : IX, Issue : VIII, August - 2020

SERUM PROTEOME ANALYSIS AND IDENTIFICATION OF SURROGATE PROTEIN MARKERS IN MALARIA

Tundwal Vijay Kumar, Satyaveer Singh, Somveer, Jinger Naveen Kumar, Meena Manoj Kumar, Kochar Aditya, Tundwal Divyanshi, Kochar Sanjay Kumar

Abstract :

Aim and objective: Serum proteome analysis of patients suffering from severe and non–severe vivax and falciparum malaria infections to get mechanistic insights about disease pathogenesis and host immune response. Validation of candidate serum protein biomarkers for P. vivax and P. falciparum infections malaria. Material & Methods: This study was carried out as a hospital – based, observational case–control study and conducted in total 8449 cases of acute feile illness with symptoms in the Department of Medicine. Proteomic and MRM assay based analysis of the clinical samples will be conducted in Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai. The study was conducted in two steps. The first step was to identify patients with P. vivax and falciparum malaria, collect clinical data and serum samples while the second step was to subject the serum samples to proteomic study with appropriate controls. 34samples were collected out of 74 for research purpose, rest of the cases were excluded due to patient refusal for consent and concomitant illnesses. 7 cases were further excluded from our study due to improper sample transportation and handling. 27 samples were processed and enrolled in our study. Results: In our study serum proteome analysis of patients suffering from severe and non–severe vivax and falciparum malaria infections was done to get mechanistic insights about disease pathogenesis and host immune response. Validation of candidate serum protein biomarkers was done for P. vivax and P. falciparum infections. Inter–alpha–trypsin inhibitor was found to be significantly down–regulated in P. vivax malaria cases. Whereas ceruloplasmin was found to be up–regulated and Cystatin–C protein was down–regulated in vivax malaria cases. Catalase, ComplementC1r subcomponent–like protein, Complement component C8 beta chain was up–regulated in P.falciparum cases. Out of these three, catalase was found to be significantly up–regulated. Conclusion: Several proteins were identified in our study, Inter–alpha–trypsin inhibitor was found to be significantly down–regulated in P. vivax malaria cases. Whereas ceruloplasmin was found to be up–regulated and Cystatin–C protein was down–regulated in vivax malaria cases. Catalase, ComplementC1r subcomponent–like protein, Complement component C8 beta chain was up–regulated in P. falciparum cases. Out of these three, catalase was found to be significantly up–regulated. Including a larger number of patients to confirm the findings obtained in this analysis is required so as to confirm the findings.