Volume : II, Issue : XII, December - 2013

Radiation induced enhancement of protein content in Soybean (Glycine max L. Merr)

Deepthi Somasekharan, Dr. K. M. Khaleel

Abstract :

Soybean samples were exposed to various doses of electron beam irradiation (50, 100, 150, 200 and 250Gy). The irradiated and non-irradiated soybean seeds were grown in greenhouse condition. Proteins were extracted from the seeds of F1 generation soybean plants and its protein content was estimated using Lowry’s method. Protein profiling of the F1 generation soybean plants of irradiated and non-irradiated seeds was done using SDS-PAGE. The results demonstrated that the total protein in the F1 soybean plants of irradiated seeds increased with the increase in the radiation dosage and was found to be higher than the seeds of non-irradiated F1 soybean plant. Also, the protein profile in F1 seeds of irradiated sample showed significant difference in the band patterns, compared to the non-irradiated. Electron irradiation seems to be a good procedure for improving the nutritional quality of soybean.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Deepthi Somasekharan, Dr. K. M. Khaleel Radiation induced enhancement of protein content in Soybean (Glycine max L. Merr) International Journal of Scientific Research, Vol.II, Issue.XII December 2013

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