OPTIMIZATION OF CULTURE CONDITIONS TO ENHANCE POLYETHYLENE ENZYME PRODUCTION BY USING PSEUDOMONAS

G.Latha; V.Ambikapathy; A.Panneerselvam

Volume : III, Issue : VI, June - 2014

OPTIMIZATION OF CULTURE CONDITIONS TO ENHANCE POLYETHYLENE ENZYME PRODUCTION BY USING PSEUDOMONAS

G. Latha, V. Ambikapathy, A. Panneerselvam

Abstract :

In the present research work, Pseudomonas was isolated from mangroves of pichavaram.Screening was performed with the help of clear zone method. LDPE act as a sole carbon source for the production of polyethylene degrading enzyme. Mineral salt medium was used for the production of enzyme. Enzyme assay was performed to nullify the producing ability of microorganisms. Protein content of the enzyme also calculated. The activity of enzyme production was optimized with the study of culture condition like, incubation time, temperature and pH. The maximum activity was showed in pH 7.5and at temperature 35? C after two days of incubation period. The goal of this work was to enhance the polyethylene degrading enzyme production by pseudomonas to be applied in the enzymatic degradation of polyethylene in future.