Optimization of culture conditions for production of xylanase from Thermomyces lanuginosus by liquid state fermentation

Seema Rawat

Volume : IV, Issue : V, May - 2015

Optimization of culture conditions for production of xylanase from Thermomyces lanuginosus by liquid state fermentation

Seema Rawat

Abstract :

Xylanase is an important enzyme having various biotechnological applications. The present work focused on optimization of culture conditions for enhanced enzyme production. The maximum production was observed at 400C (15.20?0.20 U/ml) after 96h of incubation (15.20?0.20 U/ml) and at pH 6.5 (15.20?0.20 U/ml. Maximum enzyme production was observed on wheat an as substrate (15.20?0.20 U/ml) and at 1.5% wheat an (18.10?0.10 U/ml. Maximum xylanase production was observed on yeast extract (18.10?0.10 U/ml) and at 1.5% level (20.10?0.10 U/ml). All additives and detergents decrease the activity of enzyme. Maximum decrease was observed when tween–80 was added (10.15?0.21U/ml). Ca2+ and Mn2+ were found to enhance xylanase production.