Isolation, Identification and Characterization  of cellulase producing Actinomycetes  bacterium FMR 3 (KF011478) from Slaughter  House Waste

Dr. S. Meignanalakshmi; I. Faridha Begum; S. Vinoth Kumar; J. Deepika

Volume : II, Issue : VII, July - 2013

Isolation, Identification and Characterization of cellulase producing Actinomycetes bacterium FMR 3 (KF011478) from Slaughter House Waste

Dr. S. Meignanalakshmi, I. Faridha Begum, S. Vinoth Kumar, J. Deepika

Abstract :

In the present study, slaughter house waste was collected and bacterial colonies were isolated by serial dilution method. The colonies were screened for cellulase production by Congo Red Assay. The isolate which produced maximum zone of clearance was identified as Actinomycetes bacterium by biochemical test, sugar fermentation test and 16S rRNA sequence analysis. The Actinomycetes was found to be gram positive, motile, rod shaped, filamentous, spore forming bacteria. It has fermented glucose, maltose, sucrose and starch. The identified bacterial sequence was submitted in GenBank and obtained the accession number KF011478. The phylogenetic tree analysis by neighbor-joining method also confirmed that the isolate belongs to the family Actinomycetes. Summary of the paper: The cellulase producing Actinomycetes bacterium was isolated from slaughter house waste. The isolated Actinomycetes sp. was found to be gram positive, motile, rod shaped, filamentous, spore forming bacteria. The 16S rRNA analysis was carried out and the PCR product was sequenced. The sequence was submitted in GenBank and obtained the accession number KF 011478. The phylogenetic tree was constructed by neighbor-joining method.