Volume : IV, Issue : VI, June - 2015
ISOLATION AND CHARACTERIZATION OF PHB PRODUCING Buttiauxella agrestis FROM SLAUGHTER HOUSE WASTE RUMEN FLUID
Meignanalakshmi S, Legadevi R, Sworna Kumari C, Palanisammi A
Abstract :
Buttiauxella agrestis, a PHB producing bacterium was isolated from rumen fluid collected from slaughter house waste by sudan black staining. Buttiauxella agrestis was found to be a gram negative motile rod. It was found to be positive for Methyl red, Citrate utilization test, Glucose, Arabinose, Lactose, mannitol, Rhamnose, Sucrose and negative for Indole test, Voges proskauer, Adonitol, Sorbitol test. PHB production by Buttiauxella agrestis was confirmed by acridine orange and nile blue A staining. The bacteria produced dark yellow orange colour fluorescence at 640 nm and red fluorescence at 480 nm for acridine orange and nile blue staining respectively. Buttiauxella agrestis was grown in a nitrogen deficient minimal medium(NDMM) for PHB production. PHB was extracted by chloroform–sodium hypochlorite method. PHB yield was found to be 0.56 g/L. PHB was confirmed by FTIR analysis.
Keywords :
Article:
Download PDF
DOI : 10.36106/ijsr
Cite This Article:
Meignanalakshmi S, Legadevi R, Sworna Kumari C, Palanisammi A Isolation and Characterization of PHB
Producing Buttiauxella agrestis From
Slaughter House Waste Rumen Fluid International Journal of Scientific Research, Vol : 4, Issue : 6 June 2015
Number of Downloads : 1058
References :
Meignanalakshmi S, Legadevi R, Sworna Kumari C, Palanisammi A Isolation and Characterization of PHB Producing Buttiauxella agrestis From Slaughter House Waste Rumen Fluid International Journal of Scientific Research, Vol : 4, Issue : 6 June 2015
Our Other Journals...
-
Indian Journal of
Applied Research Visit Website -
PARIPEX Indian Journal
of Research Visit Website -
Global Journal for
Research Analysis Visit Website