Volume : II, Issue : XII, December - 2013

IN VITRO POLLEN GERMINATION OF DATURA METEL L. ( 5 ) EFFECT OF HORMONES

Ms. Mital Katara, Dr. Archana Mankad

Abstract :

In vitro germination of pollen is simple and fully quntative test for assaying pollen viability and also can be exploited for study of pollen physiology. A nutrient medium (in vitro) is supplemented with some essential compounds necessary for pollen germination and tube growth. During in vitro pollen germination and tube growth not only enzyme activity but also the effect of Carbohydrates, boron, calcium, Potassium nitrate, hormones, light and other factors have been studied for different plants. Here Datura metel L. was selected. In order to get maximum germination and tube growth in this taxon, modification of the basal medium of Brewbaker and Kwack was used. Datura metel L. pollen gave good percent germination in 10^-5 M Gibberellic acid, 10^-5 M Indole-3-acetic acid, 10^-5 M Kinetin, 50 ppm Salicylic acid, 50 ppm Maleic hydrazide . As major component of pollen tube reserves hormones have a vital role to play in its germination and growth.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Ms. Mital Katara, Dr.ARCHANA MANKAD In Vitro Pollen Germination of Datura Metel L. ( 5 ) Effect of Hormones International Journal of Scientific Research, Vol.II, Issue.XII December 2013

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