Identification of Non– fermenter Gram negative bacilli (NFGNB) from various clinical isolates with special reference to Carbapenem resistance in various isolates.

Dr Anu Sharma; Dr Mukesh Kumar; Dr K.V.Ingole; Dr Ravi Prakash Sharma

Volume : VII, Issue : X, October - 2018

Identification of Non– fermenter Gram negative bacilli (NFGNB) from various clinical isolates with special reference to Carbapenem resistance in various isolates.

Dr Anu Sharma, Dr Mukesh Kumar, Dr K. V. Ingole, Dr Ravi Prakash Sharma

Abstract :

Non Fermenter Gram negative bacilli (NFGNB) has emerged as important hospital pathogens they are more significant as they are found to be multi drug resistant. Resistance to carbapenems is common among NFGNB.Aims&Objectives: To isolate & identify NFGNB from various clinical samples and to detect resistance to carbapenem in isolates resistant to Imipenem.Material & Method:NFGNB isolated from various samples were speciated using standard tests.Isolates resistant to Imipenem were subjected to detection of MBLs(Metallo–β–lactmase) and Amp–C.Results:Out of 1566 samples received, NFGNB were 200. Among them 112 were Pseudomonas aeruginosa from which 31 were found to be resistant to Imipenem, of which 3 were MBL producer by Modified Hodge test while 4 were MBL producer by EDTA disc synergy test. Out of 200 NFGNB 71 were Acinetobacter baumanii, of which 23 were found to be resistant to Imipenem, of which 6 were MBL producer by Modified Hodge test, while 4 were seen to be MBL producer by EDTA disc synergy test. Nineteen isolates of Acinetobacter baumanii were found to be resistant to cefoxitin of which 6 were found to be Amp–C producer by Amp–c disc test. None of the Pseudomonas aeruginosa were Amp–C producer. Other NFGNB isolated were either sensitive to Imipenem or if resistant were not MBL or Amp–C producer.