Expeditious Synthesis of Silver Nanoparticles  By A Novel Strain Sporosarcina pasteurii  SRMNP1 and Patrocladogram Analysis For  Exploration of its Closely Related Species

Arul Jothi Nagarajan; Sivaraj Irusappan; Gautami Amarnath; Swathine Chandrasekaran; Sruthi Abirhami B K; Janitri V Babu; Harishankar M K; Dr. Devi A

Volume : III, Issue : II, February - 2014

Expeditious Synthesis of Silver Nanoparticles By A Novel Strain Sporosarcina pasteurii SRMNP1 and Patrocladogram Analysis For Exploration of its Closely Related Species

Arul Jothi Nagarajan, Sivaraj Irusappan, Gautami Amarnath, Swathine Chandrasekaran, Sruthi Abirhami B K, Janitri V Babu, Harishankar M K, Dr. Devi A

Abstract :

Sporosarcina pasteurii is known for its calcite precipitation property and is being explored in biological cementing industries. The present study involves biological expeditious synthesis of silver nanoparticles by the bacteria in cost effective manner in less than 18 hours. The property of silver tolerance and reduction of Sporosarcina pasteurii SRMNP1 (Accession number KF214757) strain is explored for the first time. The bacteria were subjected to different concentrations of silver nitrate and maximum tolerance was observed at 20 mM. The reduction of silver nitrate was indicated by the colour change of solution from whitish to own. The resultant biomass after 18 hours of incubation was dried and lyophilized and characterized using SEM and EDAX. The analysis shows that the bacteria posses crystalline silver nanoparticles on their surface which shows the extracellular synthesis of silver by the bacteria. The study concludes that the Sporosarcina pasteurii SRMNP1 can be used as a potential source for biological synthesis of silver nanoparticles and further studies can also be made to explore the metal tolerance property of the organism. The evolutionarily close related species can also be explored for the bio-synthesis of silver nanoparticle in near future.a