Volume : VI, Issue : VII, July - 2017
Evaluation of hepatitis B and C virus seromarkers of donor’s blood using three different methods
Borhan Uddin Sheik, Dr. Rosy Sultana, Dr. Manjuma Rahman, Mohammad Moniruzzaman
Abstract :
Background: Need for blood transfusion requires no special clarification. But safe transfusion is of utmost importance. Safe blood transfusion program proposed mandatory screening of hepatitis B surface antigens (HBsAg), antibody to hepatitis B core antigen (Anti–HBc) and antibody to hepatitis C virus (Anti– HCV) of donor’s blood for transfusion by different laboratory methods. Aims and objectives: Considering the fact the present study was undertaken to estimate hepatitis B and C virus seromarkers of donor’s blood using Chemiluminescence Microparticle Immunoassay (CMIA) and Immunochromatography (ICT) and compared with Enzyme Linked Immunosorbent assay (ELISA) method. Materials and Methods: In this cross sectional study a total number of 80 blood donors were included in the study following the selection criteria. Sensitivity, specificity, negative predictive value and positive predictive value of the methods were calculated using McNemar Test. Results: Donors blood was screened for HBsAg, Anti–HBc and Anti–HCV by ELISA, CMIA and ICT methods. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of ELISA, CMIA and ICT methods were evaluated. Of the 80 samples positivity for HBsAg and Anti–HCV by CMIA was found in 1 (1.25%) and 2 (2.5%) samples respectively, whereas all 80 (100%) were negative for both the seromarkers as measured by ELISA as well as ICT. Positivity for Anti–HBc was observed in 6 (7.5%) samples by both the CMIA and ELISA methods. Specificity for CIMA in determination of HBsAg was 98.7% and NPV 100%. Sensitivity and PPV could not be calculated. Determination of anti–HBc by CMIA showed sensitivity and specificity as 100% and NPV and PPV was also 100%. The trend was similar for CMIA when considered anti–HCV. Determination of HBsAg and anti–HCV using ICT showed specificity and NPV of 100% when compared to ELISA. Sensitivity and PPV could not be calculated. Conclusions: ICT for screening of HBsAg and anti HCV showed closer result with ELISA and CMIA. This small study highlighted the promise of using the relatively less time consuming, less costly and easy to perform ICT method for screening of donor’s blood in remote setting.
Keywords :
Chemiluminescence Microparticle Immunoassay (CMIA) Immunochromatography (ICT) Enzyme Linked Immunosorbent assay (ELISA) Hepatitis B virus (HBV) Hepatitis C virus (HCV).
Article:
Download PDF
DOI : 10.36106/ijsr
Cite This Article:
Borhan Uddin Sheik, Dr. Rosy Sultana, Dr. Manjuma Rahman, Mohammad Moniruzzaman, Evaluation of hepatitis B and C virus seromarkers of donor¥s blood using three different methods, INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : VOLUME-6 | ISSUE-7 | JULY-2017
Number of Downloads : 626
References :
Borhan Uddin Sheik, Dr. Rosy Sultana, Dr. Manjuma Rahman, Mohammad Moniruzzaman, Evaluation of hepatitis B and C virus seromarkers of donor¥s blood using three different methods, INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH : VOLUME-6 | ISSUE-7 | JULY-2017
Our Other Journals...
-
Indian Journal of
Applied Research Visit Website -
PARIPEX Indian Journal
of Research Visit Website -
Global Journal for
Research Analysis Visit Website