Comparative study of biological hydrogen production using various microbial strains

Peng Chen; Lei Yan; Ning Liang; Xiaojuan Yan; Ningbo Wang; Suyue Li; Hongyu Li

Volume : III, Issue : II, February - 2014

Comparative study of biological hydrogen production using various microbial strains

Peng Chen , Lei Yan, Ning Liang, Xiaojuan Yan, Ningbo Wang, Suyue Li, Hongyu Li

Abstract :

Hydrogen is a promising alternative fuel for the future because its non-polluting and inexhaustible nature, it has high heating power and the potential to eradicate all the environmental problems. As an importantly and specially industrial gases, hydrogen has a wide application and potentiality in petrochemical, metallurgic industry, electronics, food processing, refined organic synthesis and aviation. Among the various hydrogen production pathways, use of biological methods of hydrogen production has attracted increasing global attention, due to its potential for inexhaustible, low-cost and renewable source of clean energy. The aim of this work was to observe the hydrogen production yield of 16 different pure strains and mixed culture, which contains hydrogen-producing strains and non-hydrogen-producing strains. The experimental results compared the hydrogen production yield of these pure strains. In addition, The biohydrogen production in mixed culture of Enterobacter cloacae ATCC 13047, Kluyveromyces marxianus 15D and Clostridium acetobutylicum ATCC 824 was greatly enhanced up to 33.7 mL/h^-1.L^-1, indicating that biohydrogen production in mixed culture is preferable for the fermentative hydrogen-producing system. These results confirmed Enterobacter cloacae ATCC 13047, Kluyveromyces marxianus 15D and Clostridium acetobutylicum ATCC 824 of hydrogen-producing bacteria has potential and efficient applications in biohydrogen production.