Collection, Isolation and Characterization  of Magnetotactic Bacteria From Fresh  Water Lake Sediment

Vidya A Karande; Rupali N Patil; Saral J Ghosh; Shivaji H Pawar

Volume : III, Issue : IV, April - 2014

Collection, Isolation and Characterization of Magnetotactic Bacteria From Fresh Water Lake Sediment

Vidya A Karande, Rupali N Patil, Saral J Ghosh, Shivaji H Pawar

Abstract :

Magnetotactic Bacteria (MTB) is important resource for extraction of nanomagnet. It is a major constituent of natural microbial community and plays a significant role in biomineralization. The chemical analysis and biodiversity of the Rankala lake environment have been studied. In this study, collection method was designed to isolate MTB from Rankala lake sediment based on their magnetotaxis. The obtained four isolates were cultivated in modified enrichment medium at 260 C with microaerophilic condition under the field of permanent magnet. The results of Atomic Absorption Spectroscopy (AAS) estimated the iron content of isolates is 10 times more than the nonmagnetic bacteria. The present study reports the significance of combined approach of simple magnetic assembly and modified enrichment medium for cultivation of magnetotactic bacteria. Isolation and mass cultivation of magnetotactic bacteria for magnetosomes have been great potential in nanotechnology.