Clinico–Pathological Correlation Of The  Expression Of Cyclin D1 And Bcl–2 In Oral  Squamous Cell Carcinomas –A Tissue  Microarray Study

Dr Monica Charlotte Solomon; Dr M S Vidyasagar; Dr Donald Fernandes; DrVasudeva Gudattu

Volume : III, Issue : II, February - 2014

Clinico–Pathological Correlation Of The Expression Of Cyclin D1 And Bcl–2 In Oral Squamous Cell Carcinomas –A Tissue Microarray Study

Dr Monica Charlotte Solomon, Dr M S Vidyasagar, Dr Donald Fernandes, Drvasudeva Gudattu

Abstract :

Oral squamous cell carcinomas (OSCC)containa heterogenous tumor cell population that are at different stages of differentiation and with different levels of proliferative potential. Hence, the behavioural pattern of individual cases is poorly predictable. Several molecules play a vital role in the step-wise process of oral carcinogenesis. A better understanding of the interaction of these molecules will help in identifying the subgroup of patients with poor outcomes. Aim and Objective To analyse the immunohistochemical expression of the cyclin D1 and bcl-2 in oral squamous cell carcinomas. To evaluate the possible association between cyclin D1 and Bcl-2 during the progression of oral squamous cell carcinomas. Settings and Design A systematic approach of tissue microarray technology and immunohistochemistry analysis for a cohort of oral squamous cell carcinoma patients. Materials and methods: Formalin Fixed Paraffin Embedded (FFPE) tissue blocks of histological proven cases of OSCC (n=119) were selected. Tissue microarray (TMA)paraffin blocks were constructed using a �handy tissue microarrayer�. 4 �m thick sections were cut from the TMA blocks and the sections were immunostained with cyclin D1 and Bcl2. A semiquantitative analysis of the immunohistochemical expression of these markers in the tumor cells was carried out.