Association of Paraoxonase-1 levels and G allele (192 A/G polymorphism) in occurrence and recurrence of Myocardial Infarction

Dr Ritu Singh; Ms Sana Tasnim; Dr Seema Kumari; Dr Jayashree Bhattacharjee; Dr Sanjay Tyagi

Volume : IX, Issue : VIII, August - 2020

Association of Paraoxonase-1 levels and G allele (192 A/G polymorphism) in occurrence and recurrence of Myocardial Infarction

Dr Ritu Singh, Ms Sana Tasnim, Dr Seema Kumari, Dr Jayashree Bhattacharjee, Dr Sanjay Tyagi

Abstract :

Background and objective: Oxidative stress plays an important role in atherosclerosis and myocardial infarction (MI). Due to antioxidant properties of Paraoxonase–1, we studied the implication of Paraoxonase–1 gene polymorphism (192A/G) on occurrence and recurrence of MI. Material and Methods: 200 patients of acute MI and 100 age and sex–matched controls were included. Paraoxonase–1 genotypes were evaluated by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism. Serum Paraoxonase–1 levels were estimated by ELISA. Result and Discussion: Our findings were that significantly low serum Paraoxonase–1 concentration (p–value=<0.0001) was observed in patients with acute MI as compared to controls. Allelic frequency of A was more in the population that we studied but we observed that the frequency of G allele was more in patients of MI as compared to controls. Our results further suggest that individual with GG genotype were more prone to develop MI even if there is no family history of MI thus indicating that GG genotype has significant role in development of MI. Conclusion: The G allele was more frequent in the MI patients than in the healthy subjects, and also there are significant reduced levels of Paraoxonase–1 in patients with G allele, thus suggesting that it may play a role in the pathogenesis of cardiac ischemia in our Indian population.