Volume : III, Issue : II, February - 2014

Antibacterial finish for Hospital dressing using Silver Nanoparticles from ESBL producing Pseudomonas aeruginosa

Yasodha, Dr. J. Vimalin Hena, Mamatha. C

Abstract :

In this study, Pseudomonas aeruginosa was isolated from pus samples and was characterized using phenotypic and genotypic methods. It was confirmed to be an ESBL producer through Double Disc Synergy assay. This was then used to produce silver nanoparticles using two different concentrations of silver nitrae over a period of 24 hrs. The produced nanoparticles were characterized using UV spec analysis, FTIR and SEM. The antibacterial activity of the nanoparticles was checked. These were further coated onto hospital dressing cloth and this cloth was subjected to antibacterial screeing. It had the highest absorbance at 370 nm and the synthesis was maximum when the silver solution was used at 1mM concentraton. FTIR characterization showed that the nanoparticles contained different functional groups.SEM results showed that the nanoparticles were smaller than 1µm in diameter and the smaller particles appeared at 93 nm and the larger ones appeared at 120 nm. These nanoparticles alone and also when coated on hospital dressing cloth showed high antibacterial activity.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Yasodha, Dr. J. Vimalin Hena, Mamatha.C Antibacterial finish for Hospital dressing using Silver Nanoparticles from ESBL producing Pseudomonas aeruginosa International Journal of Scientific Research, Vol.III, Issue.II February 2014

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