An outbreak of Burkholderia cepacia complex in the paediatric unit of a tertiary care hospital

Shivani; Shaveta Kataria; Anato K Swu; Neerja Jindal; Rinkal Kansal; Trimaan Kaur Bains; Rishu Lal

Volume : IX, Issue : VIII, August - 2020

An outbreak of Burkholderia cepacia complex in the paediatric unit of a tertiary care hospital

Shivani, Shaveta Kataria, Anato K Swu, Neerja Jindal, Rinkal Kansal, Trimaan Kaur Bains, Rishu Lal

Abstract :

Introduction: Burkholderia cepacia complex (Bcc) is a non fermentative Gram–negative bacillus, now increasingly recognized as an important human pathogen causing hospital acquired infections. Bcc is a common contaminant in pharmaceutical products. We have described an outeak of Bcc bacteraemia amongst children admitted in paediatric ICU and paediatric ward of tertiary care hospital in North India. Materials and Methods: The study was conducted in a department of microbiology in a tertiary care hospital of North India for a period of 6 months from 1 July 2019 to 31 December 2019. Blood culture sam–ples from paediatric patients yielded growth of non fermenting , oxidase positive, motile, gram negative bacilli which were sent to the department of Medical Microbiology, Post Graduate Institute of Medical Education and Research Chandigarh for identification by Matrix – assisted laser desorption /ionization –Time of flight– Mass Spectrometry( MALDI–TOF MS). Antimicrobial susceptibility of the clinical isolates was performed on Muller–Hinton agar by modified Kirby–Bauer�s disc diffusion method in accordance with Clinical Laboratory Standards Institute 2019 guidelines. Additional samples were collected from paediatric unit to identify the source. Results: The study involved 31(7.7%) cases of blood culture proven septicemia due to Bcc among patients admitted in paediatric ICU and ward. All the Bcc isolates showed a similar antibiogram with resistance to Amikacin, Gentamicin, Ceftazidime, Cefipime, Levofloxacin and Colistin and 100% sensitivity to Piperacillin –Tazobactam, Meropenem, Imipenem and Cotrimoxazole. Source of the out–eak could not be identified despite active surveillance. Conclusion: Early diagnosis and appropriate therapy, along with impementation of essential hospital infection control practices is required for successful containment of this pathogen.