Toxic Effect of Acrylamide on Body Weight, the  Study of Antioxidants and Histoarchitecture of Heart  in the Developing Chick Embryo

MV Swamy; KV Subbaiah; B Suman; K Kamala; KJ Rao K; Thyaga Raju

Volume : III, Issue : VII, July - 2013

Toxic Effect of Acrylamide on Body Weight, the Study of Antioxidants and Histoarchitecture of Heart in the Developing Chick Embryo

Mv Swamy, Kv Subbaiah, B Suman, K Kamala, Kj Rao K, Thyaga Raju

Abstract :

Acrylamide, used in many fields, from industrial manufacturing to laboratory work, is also formed during the heating process through the interactions of amino acids. Therefore, acrylamide poses a significant risk for both human and animal health. The present study carried out to investigate the toxic effects of acrylamide in alterations of cardiac, total body weight, antioxidant enzyme activities and histoarchitecture of heart, at the end of experiment (after 72h) the tissues were collected from the 11th day (d11) developing chick emyos exposed to different doses of acrylamide toxicity. The present results showed that catalase, superoxide dismutase, glutathione peroxidase, and glutathioneS–transferase enzymatic activities were reduced with increasing doses of acrylamide, the decreased activities antioxidant enzymes levels in discrete regions of heart indicating oxidative stress and inhibited antioxidant defense. However low doses of acrylamide significantly increases the heart and total body weights and increased the doses of the acrylamide induces the loss of heart and body weights. Light microscopic examination revealed severe structural changes in acrylamide–treated emyos. The dose of 0.2mg tissue showing mild degeneration at myocardial fibers, 0.4mg showing tissue degeneration with necrotic and degenerative changes and dose of 0.6mg showing collapsed myocardial fibers indicates degeneration of tissue. These findings concluded that high doses of acryalmide toxicity induced the oxidative stress, loss of heart and total body weight and collapsed myocardial fibers.