Synthesis of Silver Nanoparticles Using Bovine Serum  Albumin, Characterization and Their Bioevaluation

RaviShankar Kumar; Anjali Jha

Volume : III, Issue : X, October - 2013

Synthesis of Silver Nanoparticles Using Bovine Serum Albumin, Characterization and Their Bioevaluation

Ravishankar Kumar, Anjali Jha

Abstract :

A simple and convenient method is synthesis of silver nanoparticles (AgNPs) in a foam matrix using the protein bovine serum albumin (BSA) is reported. BSA is an excellent foaming agent and by virtue of its zwitterionic character at the protein isoelectric point, may be used to bind to cationic silver (Ag+) ions in the foam. The metal ions in the foam are thereafter reduced in situ to yield silver nanoparticles. The BSA molecules coat and stabilize the nanoparticles thus eliminating the necessity of employing an additional stabilizing agent in the experimental procedure. The synthesized silver nanoparticles were characterized by FTIR, UV–Vis spectroscopic and powdered x–ray diffraction studies with Silver nanoparticles of size 26nm were obtained at pH 0.5.Antibacterial studies reveal that silver nano particles shows significant inhibition on Gram negative bacteria (E. coli and Brevibacterium) than Gram positive bacteria (Streptococci & Bacillus subtilis) tested.