Volume : IV, Issue : VIII, August - 2014

Studies on Production of Antimicrobial Substances from BACILLUS Species Isolated from Lonar Lake

Tambekar Dh, Tiwari Aa, Tambekar Sd

Abstract :

The alkaline Lonar Lake is a unique ecosystem formed by meteor impact and situated in the Buldhana District, Maharashtra state (India). The development of resistance to multiple drugs is the major problem; therefore antimicrobial substances producing Bacillus sp. were isolated from the water, matt and sediment of alkaline Lonar Lake. Six Bacillus sp. were isolated using Horikoshi medium A, B, C and D. The isolated Bacillus sp. were identified by cultural, morphological, biochemical tests and by 16S rRNA gene sequencing, and found to be Bacillus fusiformis (CW4(1)), Bacillus circulance (CW2(2)), Bacillus fusiformis (CW1(3)), Bacillus cerecus (OCW3(1)), Bacillus krulwichiae (BW4(3)), Bacillus fusiformis (CW4(3)). All the selected Bacillus sp exhibited inhibitory effect against clinical isolates of pathogenic bacteria. Structural determination of the secondary metabolites was done by Gas Chromatography Mass Spectroscopy (GCMS) and results showed that OCW3(1) and CW4(1) showed strong, BW4(3) and CW4(3) showed moderate and CW1(3) and CW2(2) showed weak antimicrobial activity. Present study provides primary evidence that isolated Bacillus strains were promising sources for the antimicrobial bioactive substances and represent a new and rich source of secondary metabolites that need to be explored.

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Tambekar DH, Tiwari AA, Tambekar SD Studies on Production of Antimicrobial Substances from BACILLUS Species Isolated from Lonar Lake Indian Journal of Applied Research, Vol.4, Issue.8 August 2014

Number of Downloads : 983

References :