Simultaneous Determination of Phenolic Compounds  in Melia Azedarach. Linn Leaves by High Performance Liquid Chromatography

Mohammed Fazil Ahmed; A. Srinivasa Rao

Volume : III, Issue : XI, November - 2013

Simultaneous Determination of Phenolic Compounds in Melia Azedarach. Linn Leaves by High Performance Liquid Chromatography

Mohammed Fazil Ahmed, A. Srinivasa Rao

Abstract :

Rutin, Quercetin and Kaempferol are the most important phenolic compounds of Melia azedarach. A simple and rapid HPLC method was developed for simultaneous determination of Rutin, Quercetin and Kaempferol in ethanolic leaves extract of Melia azedarach. Acetonitrile and Phosphate buffer (pH=5.8) in ratio of 55: 45 used as mobile phase. The phenolic compounds were determined by Athena C18 column type at 254 nm with flow rate of 1 ml/min. Retention time of standards, Rutin, Quercetin and Kaempferol were found to be 2.357, 6.093 and 9.373 respectively. While the Retention times of Rutin, Quercetin and Kaempferol in Melia azedarach are 2.403, 6.143 and 8.903 which are found to be matching with standards retention time values respectively. Thus this HPLC method was found to be convenient and simple for quantitative analysis of phenolic compounds in Melia azedarach.