Volume : IV, Issue : VI, June - 2014
Silver Nitrate ParticlesNanotoxicity Using Cell Culture and Apoptosis (Genetic and Cell study)
Ragiam. Hegazy, Eman Farouk, Taghreed G. Kharboush
Abstract :
The present study focuses on the nanoparticle interaction with human peripheral blood cells from genetic and ultra structure study. AgNPsgenotoxicity and cytotoxicity had been tested in human peripheral blood lymphocytes culture through DNA fragmentation, chromosomal aberrations and electron microscope. Also, metal ion analysis was done to check the uptake of nanoparticles in the cell pellet of human peripheral blood cells. AgNPs proved to be genotoxic in human peripheral blood lymphocytes culture through the fragmentation of DNA, chromosomal aberrations and metal ion analysis. Aberrant cell damage was recorded to be 0, 8 and 24% for 5, 15 and 25μg/mL of AgNPs respectively. Significant increase and decrease in CAs and MI, respectively. Marked alterations in the ultrastructure blood cells in the form of: apoptosis of leukocytes and destruction of erythrocytes memane. Structural changes in chromosomes and chromatids were also recorded. AgNPs was found to be cytotoxic and genotoxic.
Keywords :
Silver nanoparticles blood lymphocytes genotoxicity chromosomal aberrations metaphase chromosomes hemolysis
Article:
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DOI : 10.36106/ijar
Cite This Article:
RagiaM. Hegazy, Eman Farouk, Taghreed G. Kharboush Silver Nitrate Particlesnanotoxicity using Cell Culture and Apoptosis (Genetic and Cell Study) Indian Journal of Applied Research, Vol.IV, Issue.VI June 2014
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RagiaM. Hegazy, Eman Farouk, Taghreed G. Kharboush Silver Nitrate Particlesnanotoxicity using Cell Culture and Apoptosis (Genetic and Cell Study) Indian Journal of Applied Research, Vol.IV, Issue.VI June 2014
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