Purification of Carboxylesterase Resistance to  Flubendiamide in Plutella xylostella (L.)

Dipali B. Borkar; V. L. Bagde; S. S. Munje

Volume : III, Issue : XI, November - 2013

Purification of Carboxylesterase Resistance to Flubendiamide in Plutella xylostella (L.)

Dipali B. Borkar, V. L. Bagde, S. S. Munje

Abstract :

Present investigation was undertaken to study how Carboxylesterase play significant role in flubendiamide resistance in Plutella xylostella (Diamond back moth). The resistance strain was developed upto F10 generation resistance was increased to 16.62 fold. In ammonium salt precipitation method, CarE activity was found 18.5 and 9.31 fold pure in susceptible and resistance strain respectively. In size exclusion chromatography 30–60 percent saturation fraction of fraction 1 observed high specific activity than other fraction as 0–30 and 60–100 percent.