Volume : V, Issue : V, May - 2015

Production of xylanase from Thermomyceslanuginosusby solid state fermentation

Seema Rawat

Abstract :

 Solid state fermentation (SSF) has more potential for production of enzymes especially by fungi. The present research work aimed at optimization of culture conditions for enhanced production of xylanase by  Thermomyceslanuginosus by solid state fermentation. The maximum production was observed at 400C (145.64±4.56  U/g solid) and least at 600C (10.50±2.15 U/g solid).Maximum xylanase production was observed after 96h of incubation (150.0±5.67 U/g solid) and at pH 6.5 (150.0±2.67 U/g solid) and at 1.5% wheat an (165.10±3.10 U/g solid).  Maximum xylanase production was observed on yeast extract (165.10±3.10 U/g solid) and least on potassium nitrate  (30.24±3.12 U/g solid).Maximum xylanase production was observed on 1.5% yeast extract (180.10±3.10U/g solid). All  additives and detergents decrease the activity of enzyme. Maximum decrease was observed when tween–80 was added (80.25±2.50 U/g solid).Ca2+ and Mn2+ were found to enhance xylanase production while Mg2+, Fe2+ and Cu2+  were found to decrease xylanase production.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Seema Rawat Production of xylanase from Thermomyceslanuginosusby solid state fermentation Indian Journal of Applied Research, Vol.5, Issue : 5 May 2015

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