Volume : V, Issue : IV, April - 2015
Optimization of PCR Parameters for Molecular Characterization of Gladiolus Genotypes Using ISSR Markers
Poonam Kumari, Manjunatha Rao T, Anuradha Sane, Adarsh Mn
Abstract :
Gladiolus (Gladiolus x hyidus Hort.), the queen of the bulbous ornamentals, is the leading geophyte
grown worldwide for cut flower trade and garden displays. Molecular characterization of plants relies on
high yields of pure DNA samples and optimized PCR protocol. In the present study, optimization of PCR conditions for
ISSR analysis of Gladiolus genotypes was done at Indian Institute of Horticultural Research, Bengaluru during 2011 –
2012. ISSR protocol was optimized for template DNA, dNTP, primer, and Taq polymerase concentration and annealing
temperature. Template DNA of 80 ng, 1mM of dNTP, 0.33 U of Taq DNA polymerase, 1 μM primer gave highly reproducible PCR products. Reproducible amplifiable products were observed in all PCR reactions. The present optimized
protocol for ISSR–PCR is suitable for molecular diversity analysis of Gladiolus genotypes.
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DOI : 10.36106/ijar
Cite This Article:
Poonam Kumari, Manjunatha Rao T, Anuradha Sane, Adarsh MN Optimization of PCR Parameters for Molecular Characterization of Gladiolus Genotypes Using ISSR Markers Indian Journal of Applied Research, Vol.5, Issue : 4 April 2015
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Poonam Kumari, Manjunatha Rao T, Anuradha Sane, Adarsh MN Optimization of PCR Parameters for Molecular Characterization of Gladiolus Genotypes Using ISSR Markers Indian Journal of Applied Research, Vol.5, Issue : 4 April 2015
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