Interallelic Polymorhisms of Three Different Blocks of the C– and N–Terminal of PfMSP–1 ( Plasmodium falciparum Merozoit Surface Protein 1) 2, 4 &17 from Field Isolates in South–East of Iran

A Ebrahimzadeh; TDavoodi; PEbrahimzadeh

Volume : V, Issue : IX, September - 2015

Interallelic Polymorhisms of Three Different Blocks of the C– and N–Terminal of PfMSP–1 ( Plasmodium falciparum Merozoit Surface Protein 1) 2, 4 &17 from Field Isolates in South–East of Iran

A Ebrahimzadeh, Tdavoodi, Pebrahimzadeh

Abstract :

Malaria is still a public health problem in Iran as an endemic area. Many malarial proteins have been proposed for use as a vaccine candidate antigen, but merozoite surface protein–1, Plasmodium falciparum (PfMSP–1) is the most used. It is a 180–200 kDa glycoprotein with 17 blocks from N– to C–terminal, some variable, some semi and some constant. The present study was aimed at analysing interallelic polymorphisms of MSP–1, blocks 2 , 4 ( N–terminal) and 17 (C–terminal) of three different regions, of MSP–1 gene among Plasmodium falciparum field isolates in southeast Iran. A total of 94 P. falciparum patients , were included in this descriptive cross–sectional survey. Blood samples were collected from March 2011 to September 2013. Eighty–nine cases (94.7%) of the 94 samples were successfully amplified. Genomic DNA was extracted and genetic polymorphism of PfMSP–1 blocks 2 , 4 and 17 was investigated using nested polymerase chain reactions (PCR). Two alleles of MSP–1, block 2 (K1 and MAD20) were observed and all four alleles of MSP1– block 4 genotypes and both MAD20 and K1 types of MSP–1, block 17, respectively. The block17 is one of the most important candidate region for Malaria Vaccine which obtaining MSP1–19 KDa. This area has a specific antibody epitope which plays as an inhibitor roll in invitro and invivo assay. These data are useful for malaria prevention and control in Iran.