Induction of callus and multiple shoots from nodal  cultures of Pueraria tuberosa Roxb. ex.willd.

V. Sadguna; S. Raju; T.N. Swmy; P. Karunakar Rao; Md. Mustafa

Volume : IV, Issue : I, January - 2014

Induction of callus and multiple shoots from nodal cultures of Pueraria tuberosa Roxb. ex.willd.

V. Sadguna, S. Raju, T. N. Swmy, P. Karunakar Rao, Md. Mustafa

Abstract :

Pueraria tuberosa is a wild and medicinal plant belongs to one of the largest family i.e Papilionaceae. It is growing sandy areas of the forest of southern states of India. Tuberous roots of plant are used for cardiovascular benefits and ailments of human health. Very few reports are on callus induction and no report on regeneration and somatic emyogenesis. We are attempting for clonal propagation and regeneration of plantlets form various explants, particularly from nodal cultures of endangered medicinal plant and achieved large scale callus induction and multiple shoot induction on MS medium fortified with BAP (4.0 mg/l) + IBA (0.5 mg/l) with 3.5 % sucrose in a short span of time.