IN VITRO ACTIVITY OF COLISTIN AGAINST MULTIDRUG RESISTANT GRAM NEGATIVE BACILLI (MDR GNB) ISOLATED FROM VARIOUS CLINICAL SPECIMENS

Dr. Ketaki Vyankatesh Kulkarni; Dr.Sandhya S. Kulkarni; Dr. Niranjan Pathak

Volume : VIII, Issue : IV, April - 2018

IN VITRO ACTIVITY OF COLISTIN AGAINST MULTIDRUG RESISTANT GRAM NEGATIVE BACILLI (MDR GNB) ISOLATED FROM VARIOUS CLINICAL SPECIMENS

Dr. Ketaki Vyankatesh Kulkarni, Dr. Sandhya S. Kulkarni, Dr. Niranjan Pathak

Abstract :

Introduction:Currently colistin is increasingly being used against multidrug resistant gram negative bacteria which include Pseudomonas aeruginosa,,Acinetobacterbaumanii,Escherichia coli, Klebsiellapneumoniae& Salmonella enterica. [1] Colistin resistant organisms are reported in various parts of world.Methods: Total 200 MDR GNB isolates were included in the study & tested for their susceptibility to colistin by Kirby Bauer Disc Diffusion Test.Results:Isolates consisted of 64 Escherichia coli,45 Pseudomonas aeruginosa,44 Klebsiella pneumoniae,32 Citrobacter koseri,13 Acinetobacterbaumanii& 2 Proteus mirabilis. Of these, 69 (34.5%) were resistant to Colistin. Colistin resistance in highest number was detected in Escherichia coliisolates obtained from various samples.Conclusion: As colistin resistance is emerging rapidly,measures are necessary to restrict indiscriminate use of antimicrobials& to adhere strictly to the hospital antibiotic policy. it is also the need of an hour that newer antimicrobials get discovered soon to tackle such MDR microorganisms.