Hormonal Assays of Serum FSH, LH, Prolactin, Thyroid Stimulating Hormone, Oestrogen and AMH in PCOS

Anshul Kala; Nimisha Gupta; Nita Garg

Volume : IX, Issue : III, March - 2019

Hormonal Assays of Serum FSH, LH, Prolactin, Thyroid Stimulating Hormone, Oestrogen and AMH in PCOS

Anshul Kala, Nimisha Gupta, Nita Garg

Abstract :

OBJECTIVE– The aim of this study is to estimate the levels of serum FSH(Follical Stimulating Hormone), LH(Lutenising Hormone), PRL(Prolactin), Thyroid stimulating hormone (TSH), Oestrogen(E2) and AMH(Anti Mullerian Hormone) in women with PCOS and to compare these levels with normal healthy controls. STUDY DESIGN–A hospital based cross sectional study was conducted on patients attending the Out Patient Department of Gynaecology of SGRR Institute of Medical and Health Sciences, Dehradun, Uttarakhand during a period of 1 year from August 2016 to July 2017. A total no. of 116 patients (76 cases of PCOS and 40 normal healthy controls), in the age group of 15–35 years with normal menstrual cycle were selected randomly for the study. Exclusion criteria was less than 15yrs and more than 35yr, previous history of any chronic disease like Diabetes mellitus, tuberculosis, cardiovascular diseases etc. METHODLOGY– All the 116 subjects underwent a detailed Medical history, gynaecological examination and hormonal profiling. Five milliliters of fasting venous sample was obtained in morning of 2nd/3rd day of menstrual cycle and were assayed for FSH, LH, Prolactin, TSH, Oestrogen and AMH on a fully automated analyzer Vitros 5600 of OCD. RESULTS– The serum levels of LH were significantly much higher in PCOS women as compared to normal controls. The serum levels of FSH were found to be lower in PCOS females as compared to controls. We also found a reversal in LH: FSH ratio. The serum E2 levels and AMH levels were higher in PCOS women as compared to normal females . No significant increase was found in the values of TSH and Prolactin in cases of PCOS and Normal controls, thus androgen excess in turn is responsible for many manifestations of PCOS.