Volume : III, Issue : X, October - 2013

Hepatocyte derived from Rat Bone Marrow Mesenchymal Stem Cells

Faten Zahran, Ahmed Abdel. Zaher, Nermin Raafat, Mohamed Ali

Abstract :

Hepatic Tissue Engineering is a step toward alleviating the need for organ donors. The capacity of stem cells to differentiate into specific cell types makes them very promising in tissue regeneration and repair. However, realizing this promise requires novel methods for guiding lineage–specific differentiation of stem cells. In this study we aimed to evaluate the differentiation potential of bone marrow mesenchymal stem cells (BMSCs) into hepatocytes like cells in vitro. BMSCs isolated from rat femurs and tibias were cultured at passage 3 in the presence of hepatocyte growth factor (HGF), fioblast growth factor–4 (FGF–4) and Oncostatin M (OSM). The medium was changed every 3 days and stored for albumin, alpha–fetoprotein (AFP) and urea assays. Expression of albumin, c–Met and alpha –fetoprotein mRNA were detected by reverse transcriptase –polymerase chain reaction (RT–PCR)

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Faten Zahran, Ahmed Abdel.Zaher, Nermin Raafat, Mohamed Ali /Hepatocyte derived from Rat Bone Marrow Mesenchymal Stem Cells / Indian Journal of Applied Research, Vol.3, Issue.10 October 2013

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