Volume : III, Issue : VIII, August - 2013

GC–MS screening of alklaoids of Withania somnifera L. in vivo and in vitro

Ram Avatar Sharma, Monika Goswami, Ankita Yadav

Abstract :

In this study, roots and callus of Withania somnifera (family: Solanaceae) commonly called Ashwagandha were analyzed by GC–MS for their alkaloid content. GCMS analysis confirmed the presence of 17 alkaloids in the plant and maximum was present in roots (17) > callus (13). Alkaloids present are withasomnine:(4–phenyl–1,5–timethylene pyrazole); somniferine:[1–(3–4—dimethoxybenzyl)–6–7–dimethoxyiso–quinoline]; isopelletierine: 1(2–piperidinyl)2propanone; anaferine: pyrazole[(1,3–bis,2R–piperidin–2–yl)propan–2–one)]; anahygrine: [1–amino–2(methoxymethyl) pyrrolidine]; tropine(3–end)–8–methyl–8 azabicyclo (3.2.1)octane–2–carboxylate; pseudotropine [Methyl (1R,2R,3S,5S)3–(benzoyloxy)–8–methyl–8–azabicyclo (3.2.1)octane–2–carboxylate; Iron–pseudotropine: [Fe(1R,2R, 3S,5S)–3–(benzoyloxy)8–methyl–8–azabicyclo(3.2.1)octane–2–carboxylate; withanine: 3?–glyoxy–tropane; choline; cuscohygrine; N–(3–acetylphenyl)1–Adanantanecarbox–amide; 1–[(5–Nitro–2–furfurylidene) amino]; oxacyclohexadecane–2,13–dione,13–oxime; scopoletin: Ashwagandhine:2,4–imidazolidiendione1–[{(5–nitro–2–furanyl)methylene]amino}. The alkaloid withasomine(18.12% in roots and 10.72% in callus) and anaferine(10.72% in roots and 8.14% in callus) were the predominant alkaloids in W.somnifera .

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Ram Avatar Sharma, Monika Goswami, Ankita Yadav GC-MS screening of alklaoids of Withania somnifera L. in vivo and in vitro Indian Journal of Applied Research, Vol.III, Issue.VIII August 2013

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