Evaluation of Screening, Confirmative and Reference  of Different Tests for Diagnosis of Toxoplasmosis in  Goats

Ahmed H.AL–Zuhairi; Salim H. Dhahir

Volume : III, Issue : X, October - 2013

Evaluation of Screening, Confirmative and Reference of Different Tests for Diagnosis of Toxoplasmosis in Goats

Ahmed H. Al Zuhairi, Salim H. Dhahir

Abstract :

Toxoplasmosis is an important zoonotic disease caused by the intracellular protozoan parasite Toxoplasma gondii, the disease relevant to both veterinary and human medicine. This study was designed to evaluate screening, confirmative and reference tests used for diagnosis of toxoplasma infection in goats. One hundred ninety three female goats of local eed in different status and age groups were randomly selected from five herds in Diyala province. These included 139 pregnant goats, 42 non pregnant and 14 aborted goats. Serum samples were collected and tests by latex agglutination test (LAT), enzyme linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT). Results: Higher percentage was recorded in aborted goats using three tests showed the LAT (41.66%), ELISA (25%) and IFAT (33.33%). While lower percentage was recorded in the pregnant goats by ELISA (10.79%) and IFAT (9.35%), both tests were similarly reacted in non pregnant goats. Conclusion: High correlation was observed between results of ELISA and IFAT in diagnosis of toxoplasmosis in goats.