EFFECT OF NICKEL CHLORIDE ON ANTIOXIDANT ENZYMES (SUPEROXIDE DISMUTASE AND CATALASE) IN LIVER OF Duttaphrynus melanostictus

Puspanjali Parida; Taslima Begum

Volume : VII, Issue : XII, December - 2017

EFFECT OF NICKEL CHLORIDE ON ANTIOXIDANT ENZYMES (SUPEROXIDE DISMUTASE AND CATALASE) IN LIVER OF Duttaphrynus melanostictus

Puspanjali Parida, Taslima Begum

Abstract :

Duttaphrynus melanostictus (80 g to 110 g) were divided into four groups as A, B, C and D. Eeach group comprising of five animals. Animals of group A (control) were given orally 50 µl of distilled water. The experimental animals of group B, C and D were administered orally with 50 µl (50 µg) of nickel chloride. The animals were sacrificed after different time intervals such as 0h (group A), 24h (group B), 48h (group C) and 72h (group D). The superoxide dismutase and catalase of liver at different time intervals were measured and compared. It is observed that the protein content was highest at 48 h, superoxide dismutase (SOD) level was highest at 24 h and then gradually decreases at 48 h and 72 h. The catalase (CAT) level was lower at 0 hour in comparison to 24 hour and then gradually decreased at 48 hours and 72 hours. The CAT level highest at 24 hours