Dpph Free Radical Scavenging Activity and Total  Phenolic Content of Three Species of Oyster  Mushrooms

R. Sumathy; T. P. Ajesh; R.Kumuthakalavalli

Volume : III, Issue : X, October - 2013

Dpph Free Radical Scavenging Activity and Total Phenolic Content of Three Species of Oyster Mushrooms

R. Sumathy, T. P. Ajesh, R. Kumuthakalavalli

Abstract :

Cellular damage caused by reactive oxygen species has been implicated in several diseases and hence antioxidants have significant importance in human health. In this study total phenolic content and antioxidant activity of three selected oyster mushroom species (Pleurotus florida (Mont.) Singer, Pleurotus sajor–caju (Fr.) Singer, Pleurotus citrinopileatus (Singer)) were evaluated. Methanol extract of mushrooms were analyzed for their total phenolic content by Folin–Ciocalteau Phenol method, and all the three mushrooms were found to have equal amount of phenolic content. Using phenolics as focus chemical compound, an investigation was further carried out to evaluate the antioxidant potentials of methanol and chloroform extracts of chosen mushrooms by DPPH radical scavenging assay at the concentration 100–500 mg/ml. Results revealed that the mushroom Pleurotus citrinopileatus showed higher percentage 88 –96% inhibition (for methanol) and 86–92% inhibition (for chloroform) which was concentration dependent. Percentage inhibition of Pleurotus sajor–caju and Pleurotus florida were comparatively less. The methanolic extract of all the three mushrooms showed higher antioxidant activity than the chloroform extract. These results therefore ing in new insight on these oyster mushroom species which could be used as good source of food as well as the valuable sources for drug development.