Volume : VIII, Issue : III, March - 2018
Comparison of HIV fourth generation rapid test with HIV fourth generation ELISA for screening voluntary blood donors.
Dr Satish Kumar, Dr Rajat Jagani, Dr Amit Pawar, Dr Ujjwal Dimri
Abstract :
Fourth–generation assays permit the simultaneous detection of HIV antigen and antibody. With this test, it is possible to detect HIV p24 antigen during the window period, if the blood donor happens to be tested during the short peak of high levels of circulating virus particles.
Material and Methods
In this prospective study, we screened a total of 1599 blood donors which included both whole blood donors and apheresis donors. All samples were screened with 4th generation ELISA (RFCL Limited), 4th generation Rapid Immunochromatograpy test (TRI–DOT+Ag, J. Mitra. & Co. Pvt. Ltd) and real time PCR technique.
Result and Discussion
In this study, four positive samples were detected by 4th generation ELISA, out of which two samples showed marginal positivity. All four samples were negative by 4th generation Rapid TRI–DOT +Ag, but positive with Real Time Polymerase Chain Reaction (RT–PCR) thereby implying a sensitivity of 4th gen ELISA comparable to RT–PCR, whereas sensitivity of 4th gen Rapid TRI–DOT was greatly inferior to both 4th gen ELISA and RT–PCR because of the inherent inability of the 4th gen RAPID to pick up cases of low antigenemia and low antibody titer
Conclusion
4th generation ELISA should be continued for blood donor screening and rapid TRI–DOT should not be used as an alternative to 4th generation ELISA. For a resource poor developing country like India, till the country is able to implement NAT/PCR, 4th generation ELISA is a cost effective method for blood donor screening.
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DOI : 10.36106/ijar
Cite This Article:
Dr Satish Kumar, Dr Rajat Jagani, Dr Amit Pawar, Dr Ujjwal Dimri, Comparison of HIV fourth generation rapid test with HIV fourth generation ELISA for screening voluntary blood donors., INDIAN JOURNAL OF APPLIED RESEARCH : Volume-8 | Issue-3 | March-2018
Number of Downloads : 497
Dr Satish Kumar, Dr Rajat Jagani, Dr Amit Pawar, Dr Ujjwal Dimri, Comparison of HIV fourth generation rapid test with HIV fourth generation ELISA for screening voluntary blood donors., INDIAN JOURNAL OF APPLIED RESEARCH : Volume-8 | Issue-3 | March-2018
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