COMPARING HPV DNA PCR TEST WITH HPV HC 2 FOR SCREENING OF CANCER CERVIX

Dr Jyotsna Sanjay Potdar; Dr Pushplata Naphade

Volume : VI, Issue : III, March - 2016

COMPARING HPV DNA PCR TEST WITH HPV HC 2 FOR SCREENING OF CANCER CERVIX

Dr Jyotsna Sanjay Potdar, Dr Pushplata Naphade

Abstract :

 OBJECTIVE– To compare sensitivity of HPV DNA PCR with HPV HC 2 in screening for cancer cervix. METHODSPart1– Study period for HPV DNA PCR from June 2009 to Dec 2010 at Shrimati Kashibai Navale Medical College & General Hospital in the department of Obstetrics & Gynaecology. Five hundred women underwent PAP smear exam. Twenty seven women had abnormal PAP report. Women who had abnormal PAP report had HPV DNA PCR testing. Twelve women had HSIL & Fifteen women had LSIL reports. None of these 27 women had a positive HPV DNA PCR report.(1) Part2– Study period from Jan 2012 to July 2012 at Shrimati Kashibai Navale Medical college & General Hospital where HPV DNA testing was done with HC–2 (hyid capture –2).Five hundred women underwent PAP smear. Surprisingly (as with HPV PCR test) twenty seven women had abnormal PAP report. Nineteen women had LSIL report & eight women had HSIL report. Four women of LSIL group were HPV positive ( 20% ) where as two out of eight women with HSIL report were HPV positive ( 20% ).(2)This difference between results of two studies is statistically significant with p value of less than .o1. CONCLUSION– Kit used for HPV DNA testing should be analysed before use for which detailed discussion is given for interpretation. The test kit we were using for HPV DNA PCR test had L1 as probe for test.L1 is lost during viral transformation.