Biochemical Evaluation to Mesenchymal Stem Cells  Therapy of Renal Tubulointerstitial Injury

Zahran F; El–Ghareb M; Nashwa Barakat; El–Naggar I

Volume : III, Issue : X, October - 2013

Biochemical Evaluation to Mesenchymal Stem Cells Therapy of Renal Tubulointerstitial Injury

Zahran F, El Ghareb M, Nashwa Barakat, El Naggar I

Abstract :

Background Acute renal failure (ARF) remains a frequent clinical complication, Ischemia is the major cause of acute kidney injury (AKI), associated with high mortality and morbidity. Mesenchymal stem cells (MSCs) have multilineage differentiation potential and can be a potent therapeutic option for the cure of AKI. The aim of this study was, therefore, to evaluate the therapeutic effectiveness of bone marrow–derived mesenchymal stem cells in a rat model of ischemia/reperfusion (I/R) ARF.Materials and Methods MSCs were cultured and rats were used as a common I/R model to induce ARF by clamping both renal pedicles for 45 minutes then stem cells were transplanted. Ki–67 was used as marker for regeneration of renal tubulointerstitial injury.Results This study reports that Rat MSCs decreased cell apoptosis and increased proliferation and reduced fiosis in common I/R model in rats.Conclusion MSCs may be useful in regeneration of renal tubulointerstitial injury.