Biochemical Changes During In Vitro Organogenesis  of Tylophora indica (Burm. F.) Merrill

Dipika Rathod; Asha patel; Gaurav Shrimali; Esha Rami; Chirag Patel; Jitendriya Panigrahi; Illa Patel

Volume : IV, Issue : I, January - 2014

Biochemical Changes During In Vitro Organogenesis of Tylophora indica (Burm. F.) Merrill

Dipika Rathod, Asha Patel, Gaurav Shrimali, Esha Rami, Chirag Patel, Jitendriya Panigrahi, Illa Patel

Abstract :

Biochemical changes in respect to Peroxidase, Polyphenol oxidase, IAA oxidase, Total amino acid, Reducing sugar and Protein content were studied during the process of shoot regeneration in the callus culture of Tylophora indica (Burm. F.) Merrill. The following stages: dedifferentiated callus, differentiating green callus, differentiating callus with shoot primordial, differentiating callus with well-developed multiple shoots were selected for present study. Mature leaf was used as control. From the present investigation, it was proved that the process of differentiation had high metabolic activity. High peroxidase and polyphenol oxidase and IAA oxidase activities were recorded in differentiating green callus. Protein content along with reducing sugar and total amino acid contents were decreased significantly during differentiation as compared to control. Increased metabolic rate during in vitro organogenesis help Tylophora for rapid growth and multiplication.