Assay Of Triphenylmethane Reductase  Enzyme And PCR–based Identification Of  Tmr Gene In Enterobacter Asbriae  Strain XJUHX – 4TM

Tina Mukherjee; Moumita Bhandari; Manas Das

Volume : I, Issue : V, February - 2012

Assay Of Triphenylmethane Reductase Enzyme And PCR–based Identification Of Tmr Gene In Enterobacter Asbriae Strain XJUHX – 4TM

Tina Mukherjee, Moumita Bhandari, Manas Das

Abstract :

In this study, a bacterial isolate, Enterobacter asburiae strain XJUHX–4TM, isolated from dye contaminated waste water of West Bengal (Haa), was assessed for its triphenylmethane reductase activity, the enzyme responsible for reduction based decolorization of the triphenylmethane dyes. The organism was found to demonstrate high decolorizing activity of a recalcitrant triphenylmethane dye, malachite green. The organism showed an increase in enzyme activity as the concentration of the dye –3 was increased. The maximum enzyme activity (3.586 X10 µmole/min/ml) was found at maximum concentration of the dye (500 µM) used in this study. The enzyme activity in presence of the dye became highly induced which was quite larger than the one produced from the organism in absence of the dye. The gene tmr encoding the enzyme triphenylmethane reductase was also detected which showed prominent dominance in this strain.