Antibacterial potential of alcoholic and aqueous extracts of Garcinia indica(Du Petit–Thou.) Choisy (Kokum) against clinical isolates of Clostridium difficile.

Dr. Sherin Justin; Dr. Beena Antony; Chaitra C

Volume : VIII, Issue : I, January - 2018

Antibacterial potential of alcoholic and aqueous extracts of Garcinia indica(Du Petit–Thou.) Choisy (Kokum) against clinical isolates of Clostridium difficile.

Dr. Sherin Justin, Dr. Beena Antony, Chaitra C

Abstract :

Introduction

Vancomycin and metronidazole have always been the drugs of choice for Clostridium difficile infection. Emerging resistant strains of the organism have created the need for alternative drugs. The study was intended to investigate the antibacterial potential of Kokum (Garcinia indica (Du Petit–Thou.) Choisy) extracts against clinical isolates of Clostridium difficile.

Material and Methods

The ethanolic and aqueous extracts of Kokum were prepared by soxhlation and maceration methods respectively. The screening of the extracts for their antibacterial activity was done by disc diffusion method. Minimum inhibitory concentration of the extracts was also determined by agar dilution method.

Results

All the toxigenic and non toxigenic isolates of Clostridium difficile showed sensitivity to both the Kokum extracts by disc diffusion method. The minimum inhibitory concentration range of the Kokum extracts was 2.5μl/ml to 10μl/ml.

Conclusions

Kokum extracts exhibited good in vitro antibacterial action against the clinical isolates of Clostridium difficile. This justifies the role of Kokum as a gastrointestinal remedy and could serve as an aid in development of new therapeutic options for the organism.