ANALYSIS OF BIOACTIVE COMPOUNDS INRAW (DRIED), AUTOCLAVING, BOILING AND ROASTED LINUM USITATISSIMUMSEED USING GAS CHROMATOGRAPHY AND MASS SPECTROSCOPY

S. Parameshwari; P. Nazni

Volume : V, Issue : VIII, August - 2015

ANALYSIS OF BIOACTIVE COMPOUNDS INRAW (DRIED), AUTOCLAVING, BOILING AND ROASTED LINUM USITATISSIMUMSEED USING GAS CHROMATOGRAPHY AND MASS SPECTROSCOPY

S. Parameshwari, P. Nazni

Abstract :

In this study, the bioactive components of Linum usitatissimum seeds have been evaluated using GC/MS. The chemical compositions of Linum usitatissimum seeds were investigated using Perkin–Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the plant was matched with the National Institute of Standards and Technology (NIST) liary. GC/MS analysis of raw (dried), autoclaving, boiling and roasted Linum usitatissimum seed revealed the existence of biologically active compounds.The maximum peak area represented in the raw, roasted and autoclaved linseeds were 9, 12, 15–Octadecatrienoic acid, methyl ester, (Z, Z, Z) – (88.38%) (C19H32O2) with retention time of 15.03. This compound is called as Alpha–Linolenic acid. The maximum peak area represented in the boiled linseeds was Hexadecanoic acid, methyl ester (88.33%) (C17H34O2) with retention time of 12.92. This compound is called as Palmitic acid.The results of this study offer a platform of using Linum usitatissimum seed as nutrition.