Amylase Production by ASPERGILLUS NIGER for Bioethanol Production under Solid State Fermentation

Neelam Maurya; Rekha Sirohi; Veeru Prakash

Volume : IV, Issue : IX, September - 2014

Amylase Production by ASPERGILLUS NIGER for Bioethanol Production under Solid State Fermentation

Neelam Maurya, Rekha Sirohi, Veeru Prakash

Abstract :

he delignified agro residues using 2% NaOH (sugarcane bagasse, potato peel and rice an) were used for amylase production by Aspergillus niger under solid state fermentation. The highest amylase activity was recorded with rice an at pH 5.5, temperature 400C and incubation time 120h. Resultant crude enzyme of 5.0 U/ ml was applied for hydrolysis of all pre&ndash;treated substrates in 0.1M sodium acetate buffer (pH 4.8) Hydrolysates thus obtained were used for ethanol production by inoculating Saccharomyces cerevisiae, after 48 hr maximum bioethanol was recovered.